Laser Microscope
Materials: ★★☆ Available in most school laboratories or specialist stores
Difficulty: ★★☆ Can be done by science teachers
Safety: ★★☆ Some safety precautions required to perform safely
Categories: Light, Cells and Microscopes, Microbiology
Alternative titles: Viewing Microbes with a Laser and Water Droplet
Summary
A laser is focused through a water droplet at the tip of a syringe, which acts as a convex lens. The light is projected onto a screen, allowing small aquatic organisms and impurities in the water to be observed.
Procedure
- Fill a syringe with pond water or another sample containing microorganisms.
- Place the syringe on a stand so that a droplet of water forms at the needle tip.
- Set up a white screen about 1 meter away.
- Position a laser (a laser point is sufficient) about 2 feet away from the droplet.
- Align the laser beam so it passes through the water droplet, projecting an enlarged spot on the screen.
- Gently adjust the syringe piston to maintain a stable droplet size.
- Darken the room and observe small moving shapes within the light, which show microbes and particles in the water.
Links
Laser Microscope Made With a Drop of Water - The Action Lab:
Laser Microscope - The Sci Guys:
📄 Laser Microscope Experiment - element 14: https://community.element14.com/challenges-projects/project14/provingscience/b/blog/posts/laser-microscope-experiment
Variations
- Use different light sources (red, green or blue lasers) to test visibility of microbes.
- Prepare a hay infusion by soaking hay in water for several days to cultivate microorganisms for observation.
- Compare results with filtered water to demonstrate water purity.
Safety Precautions
- Never look directly into the laser beam or point it at eyes.
- Handle syringes with care; avoid accidental pricks from the needle.
- Perform the experiment on a stable surface to prevent spills.
- Wash hands after handling pond water or other biological samples.
- Keep laser devices out of reach of young children.
Questions to Consider
- Why does the water droplet act like a convex lens?
- How does the laser light interact with microorganisms to make them visible?
- Would a microscope using visible white light show clearer details than the laser method? Why?
- How might the wavelength (color) of the laser affect what you see?
- What are some limitations of this laser microscope compared to standard optical microscopes?