Use of Volumetric Pipette

Correct technique for using a glass volumetric pipette.

• Refer to links for correct use of the volumetric pipette.

Using a volumetric pipette correctly - Michael Seery:

The Volumetric Pipet and Pipetting Technique - NAIT Chemical Technology:

📄 Using Calibrated Glass Pipettes - Lisa Nichols: https://chem.libretexts.org/Bookshelves/Organic_Chemistry/Organic_Chemistry_Lab_Techniques_(Nichols)/07%3A_Technique_Summaries/7.02%3A_Using_Calibrated_Glass_Pipettes

None

• Never pipette by mouth - always use a pipette bulb.
• Avoid drawing liquid into the bulb to prevent contamination.
• Handle glass pipettes carefully to prevent breakage and injury.
• Dispose of pipettes properly if they become contaminated with hazardous solutions.

• Why is it important not to blow out a TD pipette? (They are calibrated to deliver the correct volume without forcing out the last drop.)
• What is the difference between a TD and a TC pipette? (TD delivers a specified volume, while TC is calibrated to contain a volume that must be completely expelled.)
• Why should a pipette be conditioned with the reagent before use? (It prevents dilution or contamination from residual liquid inside the pipette.)
• How can incorrect pipetting technique affect experimental results? (It can lead to inaccurate measurements and errors in solution concentration.)