======Wet Mount Microscope Slide====== **Materials: **{{$demo.materials_description}}\\ **Difficulty: **{{$demo.difficulty_description}}\\ **Safety: **{{$demo.safety_description}}\\ \\ **Categories:** {{$demo.categories}} \\ **Alternative titles:** Mounting Living Specimens on Slides ====Summary==== {{$demo.summary}} ====Procedure==== -Place a clean glass slide on a flat surface. -Use a pipette to add a drop of your liquid sample (or place a dry specimen and then add a drop of water). -Hold a coverslip with tweezers and lower it at an angle so one edge touches the liquid. -Slowly lower the rest of the coverslip to avoid trapping air bubbles. -Use the edge of a paper towel to gently absorb any extra liquid from the sides of the coverslip. -Adjust with tweezers if needed, ensuring the coverslip is flat and stable. ====Links==== How To Prepare A Wet Mount Slide - Mr. Fox's Science Classroom: {{youtube>RfaSyfDBQzU?}}\\ 📄 How to mount your own wet microscope slides - microscopes.com.au: [[https://microscopes.com.au/blogs/news/how-to-mount-your-own-wet-microscope-slides?srsltid=AfmBOopMbxBWLnmUteqXZqFdZJ5G00Pm9FBJIlyUBgw6qMYOcaxLVR8A]]\\ 📄 Step-by-Step Procedures to Prepare Microscopic Slides - Rubicon Science: [[https://rubiconscience.com.au/preparing-microscopic-slides-wet-dry-and-heat-fixed/]]\\ ====Variations==== *None ====Safety Precautions==== *Handle glass slides and coverslips carefully to avoid cuts. *Dispose of biological samples properly. *Clean the microscope lenses and stage after use. ====Questions to Consider==== *Why is it important to lower the coverslip at an angle? (It prevents air bubbles from forming under the coverslip.) *What is an advantage of a wet mount slide compared to a permanent slide? (It allows viewing of living specimens in real time.) *Why might you seal the coverslip to the slide? (To slow evaporation and keep the sample stable.) *What might happen if you add too much water to the slide? (The specimen may move too much or drift out of focus.)