======Use of Volumetric Pipette====== **Materials: **{{$demo.materials_description}}\\ **Difficulty: **{{$demo.difficulty_description}}\\ **Safety: **{{$demo.safety_description}}\\ \\ **Categories:** {{$demo.categories}} \\ **Alternative titles:** Techniques for Accurate Liquid Transfer ====Summary==== {{$demo.summary}} ====Procedure==== * Refer to links for correct use of the volumetric pipette. ====Links==== Using a volumetric pipette correctly - Michael Seery: {{youtube>yL5XZhrWZ6I?}}\\ The Volumetric Pipet and Pipetting Technique - NAIT Chemical Technology: {{youtube>HC44xjs7dho?}}\\ 📄 Using Calibrated Glass Pipettes - Lisa Nichols: [[https://chem.libretexts.org/Bookshelves/Organic_Chemistry/Organic_Chemistry_Lab_Techniques_(Nichols)/07%3A_Technique_Summaries/7.02%3A_Using_Calibrated_Glass_Pipettes]]\\ ====Variations==== None ====Safety Precautions==== *Never pipette by mouth - always use a pipette bulb. *Avoid drawing liquid into the bulb to prevent contamination. *Handle glass pipettes carefully to prevent breakage and injury. *Dispose of pipettes properly if they become contaminated with hazardous solutions. ====Questions to Consider==== *Why is it important not to blow out a TD pipette? (They are calibrated to deliver the correct volume without forcing out the last drop.) *What is the difference between a TD and a TC pipette? (TD delivers a specified volume, while TC is calibrated to contain a volume that must be completely expelled.) *Why should a pipette be conditioned with the reagent before use? (It prevents dilution or contamination from residual liquid inside the pipette.) *How can incorrect pipetting technique affect experimental results? (It can lead to inaccurate measurements and errors in solution concentration.)