======Termite Guts====== **Materials: **{{$demo.materials_description}}\\ **Difficulty: **{{$demo.difficulty_description}}\\ **Safety: **{{$demo.safety_description}}\\ \\ **Categories:** {{$demo.categories}} \\ **Alternative titles:** Termite Dissection ====Summary==== {{$demo.summary}} ====Procedure==== - Obtain a few worker termites from a colony or commercial source. - Prepare a clean microscope slide and a drop of saline solution. - Place a termite on the slide and, using fine forceps, gently grasp the tip of its abdomen. - Carefully pull to extract the hindgut from the termite’s abdomen; it should appear as a whitish tube. - Place the hindgut into the drop of saline and gently tease it open to release the gut contents. - Add a cover slip and observe under a microscope, starting at low magnification. - Identify and sketch the various protists visible in the sample. ====Links==== What's Inside a Termite's Gut? - Walter Reed Army Institute of Research: {{youtube>2g_oZ0b8ZSM?}}\\ ====Variations==== * Compare protists from different termite species to see variation in gut symbionts. * Use stains such as methylene blue to highlight cellular features. ====Safety Precautions==== * Wear gloves and safety goggles when handling termites and dissecting tools. * Dispose of biological materials according to school or institutional biosafety guidelines. * Clean and disinfect all surfaces and tools after use. * Do not release termites or biological waste into the environment. ====Questions to Consider==== * What role do the protists play in the termite’s digestion? (They produce enzymes that break down cellulose into simpler sugars the termite can absorb.) * Why can’t termites digest wood on their own? (They lack the necessary enzymes and depend on the protists for cellulose breakdown.) * What might happen to a termite if its gut protists were lost? (The termite would be unable to digest wood and would eventually starve.) * How does this relationship illustrate symbiosis? (It is a mutualistic relationship where both organisms benefit—the termite gets nutrients, and the protists gain a habitat and food source.)