======Strawberry DNA Extraction====== **Materials: **{{$demo.materials_description}}\\ **Difficulty: **{{$demo.difficulty_description}}\\ **Safety: **{{$demo.safety_description}}\\ \\ **Categories:** {{$demo.categories}} \\ **Alternative titles:** See DNA with the Naked Eye ====Summary==== {{$demo.summary}} ====Procedure==== - Place strawberries in a resealable plastic bag and mash them thoroughly. - Add dishwashing liquid and salt to the bag and mix gently to release DNA from the cells. - Pour the mixture through a strainer or cheesecloth into a clear container. - Slowly add cold alcohol (such as isopropyl or ethanol) to form a layer on top of the strawberry liquid. - Wait a few minutes for white, stringy DNA to appear at the boundary between the two layers. - Use a stick or pipette to gently lift and collect the visible DNA strands. ====Links==== How to extract DNA from strawberries - National Human Genome Research Institute: {{youtube>hOpu4iN5Bh4?}}\\ DIY Science: How to Extract DNA from a Strawberry - University of Leicester: {{youtube>JofXXyFZn38?}}\\ 📄 Strawberry DNA extraction activity - The University of Queensland: [[https://imb.uq.edu.au/strawberry-dna-extraction-activity]]\\ ====Variations==== * Try extracting DNA from other fruits such as kiwis or bananas. * Compare how well different detergents or soaps work. * Test the effect of using cold versus room-temperature alcohol. ====Safety Precautions==== * Wear safety glasses to protect eyes from splashes. * Handle alcohol carefully and keep away from flames. * Wash hands thoroughly after the experiment. * Dispose of materials safely - do not consume the mixture. ====Questions to Consider==== * Why does detergent help release DNA from cells? (It breaks down cell membranes, releasing the contents.) * What role does salt play in the process? (It prevents proteins from sticking to the DNA.) * Why is alcohol necessary for this experiment? (DNA is insoluble in alcohol, so it clumps together and becomes visible.) * Why are strawberries particularly good for DNA extraction? (They are soft, easy to mash, and have multiple sets of chromosomes, providing lots of DNA.)