======Pineapple Enzymes and Geletin====== **Materials: **{{$demo.materials_description}}\\ **Difficulty: **{{$demo.difficulty_description}}\\ **Safety: **{{$demo.safety_description}}\\ \\ **Categories:** {{$demo.categories}} \\ **Alternative titles:** Enzymes in Action ====Summary==== {{$demo.summary}} ====Procedure==== - Open a can of pineapple pieces and set aside. - Cut fresh pineapple into pieces similar in size to the canned ones. - Place several pieces of fresh pineapple in one cup and canned pineapple in another. Label if needed. - Prepare jelly crystals with boiling water according to packet instructions. - Pour the jelly mixture into the cups with fruit, and also into plain cups with no fruit. - Refrigerate until set. Compare whether the jelly with fresh pineapple, canned pineapple, or no fruit has set properly. ====Links==== Pineapple Enzymes and Gelatin - The Sci Guys: {{youtube>7t7v8w7EqTM?}}\\ 📄 DIY Science – Enzymes in Action - National Science Week 2023: [[https://www.scienceweek.net.au/wp-content/uploads/2023/06/Enzymes-in-Action.pdf]]\\ ====Variations==== * Try adding other fruits such as kiwi, papaya, or mango to the jelly to test if they affect setting. * Cook fresh pineapple before adding it to jelly to see if the enzyme is destroyed by heat. ====Safety Precautions==== * Adult supervision required when cutting pineapple with a knife. * Adult must handle boiling water when preparing jelly. * Avoid touching eyes after handling pineapple. ====Questions to Consider==== * Why does fresh pineapple prevent jelly from setting, while canned pineapple does not? (Fresh pineapple contains bromelain, a protease enzyme that digests proteins like gelatine. Canning destroys the enzyme with heat, allowing the jelly to set.) * Why does fresh pineapple prevent jelly from setting, while canned pineapple does not? (Fresh pineapple contains bromelain, a protease enzyme that digests proteins like gelatine. Canning destroys the enzyme with heat, allowing the jelly to set.) * What other foods contain enzymes that affect proteins? (Kiwi, papaya, fig, and mango contain proteases that can prevent jelly from setting.)