======Mitosis in Onion Root Cells====== **Materials: **{{$demo.materials_description}}\\ **Difficulty: **{{$demo.difficulty_description}}\\ **Safety: **{{$demo.safety_description}}\\ \\ **Categories:** {{$demo.categories}} \\ **Alternative titles:** Cell Cycle Phase Identification ====Summary==== {{$demo.summary}} ====Procedure==== - Access digitized microscope images of onion root tips. - Review the five phases of the cell cycle: interphase, prophase, metaphase, anaphase, and telophase. - Examine the cells in the images, identifying which stage each cell is in. - Record the number of cells observed in each stage. - Calculate the percentage of cells in each phase. - Use these percentages to estimate the relative time cells spend in each stage of the cell cycle. ====Links==== 📄 Online Onion Root Tips - The Biology Project - Cell Biology: [[https://biology.arizona.edu/cell_bio/activities/cell_cycle/cell_cycle.html]]\\ ====Variations==== * Compare different regions of the root (e.g., tip vs. elongation zone) to see differences in mitotic activity. * Use prepared slides of onion root tips under a classroom microscope to replicate the digital activity. * Extend the activity to compare plant and animal cells undergoing mitosis. ====Safety Precautions==== * No safety concerns when using the online digital activity. * If replicated in the lab with real onion roots, handle stains, sharp tools, and microscopes safely. ====Questions to Consider==== * Why are onion root tips good for studying the cell cycle? (They are regions of active growth, with many cells dividing.) * Why do scientists divide the cell cycle into phases even though it is continuous? (It makes it easier to describe and study distinct events.) * Which stage of the cell cycle usually takes the longest, and why? (Interphase, because the cell is growing and preparing for division.) * How does the proportion of cells in each stage relate to the relative duration of that stage? (The more cells observed in a stage, the longer the cell spends in that stage.)