======DNA Extraction From Kiwi Fruit======


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**Alternative titles:** Kiwi Fruit DNA Extraction
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====Summary====                   
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====Procedure====  
  - Peel the skin from half a kiwi fruit and mash the fruit into a pulp.  
  - Mix in a teaspoon of salt and a small volume of washing-up liquid.
  - Filter the mixture and collect the filtrate (the liquid that passes through).  
  - Place the filtrate in an ice bath to cool.  
  - Carefully pour chilled ethanol onto the top of the cooled filtrate.  
  - Observe the formation of DNA strands at the boundary between the filtrate and ethanol.  




====Links==== 
DNA extraction from kiwi - Milne’s Science Tech: 
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📄 Extracting DNA from fruit - BBC Bitesize: [[https://www.bbc.co.uk/bitesize/guides/z3mbqhv/revision/4]]\\


====Variations====
  * Try using other fruits such as strawberries, bananas, or papayas.  
  * Compare the amount of DNA produced by different fruits.



====Safety Precautions====
  * Use ethanol in a well-ventilated area and keep away from open flames.  
  * Wear safety glasses and gloves when handling chemicals.  
  * Dispose of waste materials properly after the experiment.




====Questions to Consider====
  * What role does the detergent play in the experiment? (It breaks down cell membranes to release DNA.)  
  * Why is salt added to the mixture? (It helps the DNA clump together and become visible.)  
  * Why is ethanol used in this experiment? (DNA is insoluble in ethanol, so it precipitates out and can be seen.)  
  * Why must the ethanol be cold? (Cold ethanol improves the precipitation of DNA and makes it more visible.)